Fig. 6: Circadian rhythms in calcium localisation co-ordinate rhythms in antigen processing.

a Confocal microscopy analysis of calcium localisation in synchronised Bmal1^+/+ and Bmal1^−/− BMDCs by staining with Fluo-4 (cytosolic) or Rhod-2 (mitochondrial). b Mitochondrial calcium quantification and c cytosolic calcium quantification from confocal analysis (n = 10 independent images). d Bmal1^+/+ and Bmal1^−/− BMDCs were synchronised and pre-treated with Mdivi-1 (10 μM). Mitochondrial calcium uptake was quantified using Rhod-2 by confocal microscopy at 24 h post synchronisation (n = 28–50 independent images). e Bmal1^+/+ and Bmal1^−/− BMDCs were synchronised and treated with FK506 (12 h; 1 μM) and mitochondria morphology quantified by confocal microscopy at 24 h post synchronisation (n = 3 biologically independent samples). f Bmal1^+/+ and Bmal1^−/− BMDCs were synchronised and antigen processing was quantified by confocal microscopy at indicated timepoints by the addition of DQ-OVA (1 µg/mL) in the presence or absence of FK506 (1 µM). (n = 50 independent images). Data shown is mean with error bars representing ± SEM. Data were analysed by one-way ANOVA with Tukey’s post-hoc test for multiple comparisons. *p < 0.05, **p < 0.01 and ****p < 0.0001. Source data are provided as a Source Data file.