Fig. 2: Characterization of melanoma brain metastases (MBM) revealed different molecular subtypes.
From: Decoding molecular programs in melanoma brain metastases
a Representation of the core study cohort, including MBM that have been profiled by RNAseq, 805k global methylome analysis and/or TargetSeq. Concordance (Pt.8, 23, 24), intracranial site of tumors and the type and variant frequency (VAF) of BRAF/NRAS mutations is shown. b Unsupervised analysis of the top1000 variably expressed genes among all MBM (n = 16), compared with brain controls (BC, n = 3) revealed clustering independent from intracranial sites of discordant MBM or genetic state. Concordant MBM (Pat 23) show a convergent clustering. Legends provide information about the category (MBM, BC) or genetic state (BRAF/NRAS mutant) or intracranial site. Expression of Ecad or NGFR or markers of BTE cells such as microglia1 (TMEM119), astrocytes2 (GJB2), and neurons3 (ROBO2) among samples is indicated. c Heat map illustration of DEGs among BC and MBM, top-expressed, MBM-related genes are indicated. In b and c only significantly regulated genes (Bonferroni corrected, p < 0.05) are shown.
