Fig. 2: The Myb -68 enhancer is active in Ly6C- GMPs in Myb -68 GFP transgenic mice.
From: A Myb enhancer-guided analysis of basophil and mast cell differentiation
GFP activity in whole bone marrow (a), LS-K cells (b), and GMPs (c) of Myb -68 GFP mice. FACS gating strategies are shown in Supplementary Fig. 12. Representative FACS plots of indicated cells from control (grey) and Myb -74 GFP mice (green) are shown. Numbers shown are mean percentages±SD of GFP+ cells in Myb -68 GFP mice. The percentages of GFP+ cells are also shown in the right graphs. a n = 10 mice for LSK cells and LS-K cells, and n = 4 mice for others. p = 4.1 × 10−4 by one-way Welch’s ANOVA. ***p < 0.001 by the Games-Howell post hoc test. p = 4.1 × 10−4 between LSK and LS-K, p = 3.7 x 10−4 between CD11b+Gr1+ and LS-K, p = 3.5 x 10−4 between B220+ and LS-K, p = 3.5 x 10−4 between CD4+ and LS-K, p = 3.5 x 10−4 between CD8+ and LS-K, and p = 3.9 × 10−4 between Ter119+- and LS-K. (b) n = 6 mice. p = 5.8 × 10−7 by one-way Welch’s ANOVA. **p < 0.01 by the Games-Howell post hoc test. p = 0.0014 between PreGM and GMP, p = 0.0008 between PreMegE and GMP, p = 0.0007 between PreCFU-E and GMP, p = 0.0006 between CFU-E/ProEry and GMP, and p = 0.0006 between MkP and GMP. c n = 10 mice for Ly6C- GMPs and n = 7 mice for others. p = 8.5 × 10−6 by one-way Welch’s ANOVA. ***p < 0.001 by the Games-Howell post hoc test. p = 1.1 × 10−5 between Ly6C- GMPs and GP, and p = 1.1 × 10−5 between Ly6C- GMPs and MP. LSK Lin-Sca1+cKit+ cells, LS-K Lin-Sca1-cKit+ cells, MkP megakaryocyte progenitor, GMP granulocyte/monocyte progenitor, PreGM pre-granulocyte/macrophage, CFU-E colony-forming unit-erythroid, ProEry pro-erythrocytes, PreCFU-E pre-colony-forming unit-erythroid, PreMegE pre-megakaryocyte/erythrocyte, GP granulocyte progenitor, MP monocyte progenitor.
