Fig. 3: The Myb -68 enhancer function persists only in basophils and mast cells.

a mRNA expression levels of stem/progenitor and lineage marker genes in GFP^-Ly6C^- GMPs (grey), GFP⁺Ly6C^- GMPs (green), granulocyte progenitors (GP, blue) and monocyte progenitors (MP, yellow) (n = 5 mice for Myb, Gata2, Tal1, Apoe, and Csf3r, n = 4 mice for Csf1r and Irf8, and n = 3 mice for others). FACS gating strategies are shown in Supplementary Fig. 12 and Fig. 2c. Data are presented as mean values ± SD. p = 0.10, 0.018, 0.0016, 0.044, 0.032, 0.040, 0.0066, 8.9 × 10⁻⁵, 9.3 × 10⁻⁵, 0.27, 0.013, 2.8 × 10⁻⁷, and 1.0 × 10⁻⁷ from left to right by two-tailed Student’s t-test without adjustments for multiple comparisons. GFP activity in FcεR1α^- and FcεR1α⁺ Ly6C^- GMPs (b), basophils in peripheral blood (c), and mast cells in the peritoneal cavity (d) of Myb -68 GFP mice. FACS gating strategies are shown in Supplementary Fig. 12, 13c and 13d. Representative FACS plots of indicated cells from control (grey) and Myb -74 GFP mice (green) are shown. Numbers shown are mean percentages±SD of GFP⁺ cells in Myb −68 GFP mice. The percentages of GFP⁺ cells are also shown in the right graphs. Data are presented as mean values±SD. b n = 5 mice. ^**p = 0.0014 by two-tailed Student’s t-test. c Lin^- indicates CD4^-CD8^-B220^-Gr1^-Ly6C^-CD11c^-cKit^-Ter119^-SiglecF^-IL7Ra^-NK1.1^-. n = 4 mice for basophils and n = 5 mice for others. p = 0.0041 by one-way Welch’s ANOVA. ^*p < 0.05 by the Games-Howell post hoc test. p = 0.032 between neutrophils (Neu) and basophils (Baso), p = 0.033 between eosinophils (Eo) and Baso, p = 0.033 between monocytes (Mono) and Baso, p = 0.032 between B220⁺ cells and Baso, and p = 0.033 between CD3⁺ cells and Baso. d n = 5 mice. e Analysis of published ChIP-seq data demonstrates that multiple transcription factors including MITF bind to the Myb -68 enhancer (red rectangle).