Fig. 4: The Myb -68 enhancer enriches basophil/mast cell-biased GMPs before FcεR1α surface expression.
From: A Myb enhancer-guided analysis of basophil and mast cell differentiation
Representative FACS plots of GFP- (upper) and GFP+ (lower) Ly6C-FcεR1α- GMPs cultured in vitro in the presence of GM-CSF or IL-3 for indicated periods of time. FACS gating strategies are shown in Supplementary Figure 14a. Numbers shown are mean percentages±SD of basophils, defined as FcεR1α+c-kit- (blue), and mast cells, defined as FcεR1α+c-kit+ (red). The percentages of basophils and mast cells derived from GFP- (grey) and GFP+ (green) Ly6C-FcεR1α- GMPs are also shown in the right graph (n = 4 biological replicates from 2 independent experiments). p = 3.8 × 10−4, 1.6 × 10−3, 2.3 × 10−5, 1.0 × 10−4, and 6.9 × 10-4 from left to right by two-tailed Student’s t-test without adjustments for multiple comparisons.
