Fig. 8: Integration of two scRNA-seq datasets identified bone marrow cells with a gene signature of immature mast cells.

a UMAP visualization of in vitro mast cell culture. FACS-sorted Myb -68 GFP⁺ FcεR1α^-Ly6C^- GMPs were cultured with IL-3 for 3 days, and then analyzed. Clusters were annotated according to the expression of known feature genes. b UMAP projection of selected mast cell signature genes. c UMAP projection of the mast cell gene signature (upper) and selected mast cell genes (lower). The top 10 marker genes to characterize immature mast cells (upper right) were determined using scRNA-seq data of in vitro mast cell culture, and enrichment of the gene signature in bone marrow GFP⁺ cells in Myb -68 GFP mice was calculated by gene set variation analysis. Cells with enrichment of the mast cell gene signature were shown in red. Cells were displayed in order of expression to prevent cells with high expression from getting buried.