Fig. 9: Ly6C^- GMPs with very high expression of Integrin β7 have intracellular Granzyme b and Scinderin.

a Granzyme b expression in GMPs (left) and Ly6C^- GMPs (right) analyzed by FACS. FACS gating strategies are shown in Supplementary Fig. 14b. The left lower histogram compares the expression of Granzyme b between Integrin β7^high Ly6C^- GMPs (red, those ranked in the top 1.5% of all Ly6C^- GMPs) and other Ly6C^- GMPs (grey). The percentages of Granzyme b⁺ cells are also shown in the right lower graph. n = 2 mice. b Integrin β7^- (upper) and Integrin β7^high (top 1.5%, lower) Ly6C^- GMPs were stained with anti-Scinderin antibody (green) and Hoechst (blue). FACS gating strategies are shown in Supplementary Fig.12. Three representative cells for each from 2 independent experiments are shown. Bar shows 5 μm.