Fig. 3: Optimization of the degradation system and visualization of hcPET film degradation. | Nature Communications

Fig. 3: Optimization of the degradation system and visualization of hcPET film degradation.

From: Biodegradation of highly crystallized poly(ethylene terephthalate) through cell surface codisplay of bacterial PETase and hydrophobin

Fig. 3

a Effect of temperature, b pH, and c protein concentration on PET hydrolysis. n = 3 independent experiments. Data were presented as mean values ± SD. d Comparison of the turnover rate of the codisplay cells and purified PETase under the optimal conditions using PET as a substrate. n = 3 independent experiments. Data were presented as mean values ± SD. e SEM image of commercial hcPET film before and after incubation with PETase and codisplay cells. (Scale bar: 5 μm). f Microscopic observation of a cross-section of a commercial hcPET film before and after incubation with PETase and codisplay cells. (Scale bar: 100 μm). g Measurement result of the cross-section of the commercial hcPET film. Data were presented as mean values ± SD. All the experiments are repeated three times independently, with similar results obtained. One representative is shown for (e) and (f). Source data for panels (a–e) and (g) are provided as a Source Data file.

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