Fig. 2: Critical role of sugar-binding capacity in ThC-dependent MPL activation.
From: A marine sponge-derived lectin reveals hidden pathway for thrombopoietin receptor activation
A Relative cell proliferation in the presence of various sugars (10 mM) in Ba/F3-HuMpl cells with rThC (1 μg/mL). n = 3 per data point, bars ± SD. One-way ANOVA followed by a Dunnett’s test for multiple group comparison. B Concentration-dependent inhibition of fucose or mannose on cell proliferation by rThC treatment (1 μg/mL). IC50 for fucose and mannose were 22.8 (95% CI of 21.7–23.9), 6460 (95% CI of 6117–6842) μM, respectively. C Binding capacity of rThC to fucose- or mannose-immobilizing resins in the presence of 1 mM CaCl2 or 1 mM EDTA. Unbound, U and bound, B. Three independent experiments were carried out and similar results were obtained. D Thermodynamic analysis of the interaction with fucose in the absence (left) and presence (right) of 5 mM CaCl2. The thermogram (top) and titration curve (bottom) are shown.
