Fig. 4: Smc5/6 stably binds to replication forks.
From: Smc5/6’s multifaceted DNA binding capacities stabilize branched DNA structures
a Schematic showing a forked DNA tethered between two beads under LF. The intrinsic fork is located one quarter distance of the total tether length from the proximal bead. b Representative kymograph of a forked DNA tether in the presence of 5 nM Cy3-Smc5/6 (green). Stable binding of Smc5/6 at the intrinsic fork position was observed on 10 out of 22 (45%) tethers tested. c Quantification of the Smc5/6 fluorescence signals at the intrinsic fork (marked by the black arrow in panel b) over time. Data points indicate the averaged photon count per frame (n = 10 frames) at the fork site and error bars represent standard deviation. d Smc5/6 fluorescence intensity ratio between two timepoints (T1 and T2 as indicated by orange arrows in panel b) at an intrinsic fork under LF versus at a DNA junction generated at HF. Bar heights indicate the group mean and error bars represent standard deviation. P value was determined from a two-tailed unpaired t-test with Welch’s correction (****P < 0.0001). The sample sizes are: JunctionHF (n = 10) and Intrinsic ForkLF (n = 10), where n indicates the number of Smc5/6 streaks analyzed. Source data for panels c and d are provided within the Source Data file.
