Fig. 5: Smc5/6 assembles on free ssDNA near junctions.
From: Smc5/6’s multifaceted DNA binding capacities stabilize branched DNA structures
a Representative kymograph of a λ DNA tether being stretched from LF to HF in the presence of 20 nM Cy3-Smc5/6 (green) and 10 nM LD650-SSB (red) without ATP. The green laser was on for the entire duration of the kymograph whereas the red laser alternated between on and off. b Quantification of the SSB and Smc5/6 fluorescence signals (normalized to the first timepoint) averaged from 18 junction DNA sites such as those indicated by the black arrows in panel a. Source data are provided within the Source Data file.
