Fig. 6: Smc5/6 stabilizes DNA junctions.
From: Smc5/6’s multifaceted DNA binding capacities stabilize branched DNA structures
a Representative kymograph of a λ DNA tether alternating between LF and HF in the presence of 10 nM LD650-RPA (red). RPA was ejected from the DNA in the transition from HF to LF (examples indicated by white arrows) due to the re-annealing of complementary ssDNA. b Representative kymograph of a λ DNA tether alternating between LF and HF in the presence of 10 nM LD650-RPA (red), 20 nM Cy3-Smc5/6 (green), and 2 mM ATP. The RPA signals remained on the DNA throughout pulling-relaxation cycles (examples indicated by white arrows). c Quantification of the RPA fluorescence signals (from the individual streaks indicated by the white arrows in panels a and b) over time in the absence or presence of Smc5/6. LF and HF regimes are indicated by blue and red bars, respectively. Data points indicate the averaged photon count per frame (n = 10 frames) of a region along the RPA streak and error bars represent standard deviation. Source data are provided within the Source Data file. d (Top) Summary of the different Smc5/6 binding behaviors on DNA observed in our study. (Bottom) Working model for Smc5/6 binding modes on different types of DNA.
