Fig. 4: Generation of mosaic E2E1 nanoparticles. | Nature Communications

Fig. 4: Generation of mosaic E2E1 nanoparticles.

From: Induction of cross-neutralizing antibodies by a permuted hepatitis C virus glycoprotein nanoparticle vaccine candidate

Fig. 4

a Six E2E1-I53-50A trimers are combined and mixed with I53-50B pentamer to co-assemble as mosaic E2E1-NPs (top) or are individually mixed with I53-50B to assemble as monovalent E2E1-NP (bottom). b SEC profiles from a Superose 6 column of the mosaic E2E1-NP compared to six monovalent E2E1-NPs and the corresponding E2E1-I53-50A trimers. c NS-EM 2D class averages of mosaic and monovalent E2E1-NPs. d Binding of monovalent and mosaic E2E1-NPs to different mAbs measured by BLI. e B cell activation by E2E1-I53-50A trimers or E2E1-NPs. Activation was determined by measuring calcium flux in Ramos a B cell line harboring the AR3C bNAb. At 30 s, E2E1-I53-50A trimers or E2E1-NPs were added and calcium flux was measured after 20 s. SARS-CoV-2 spike nanoparticle (see ref. 48) and ionomycin were used as negative and positive control, respectively. Source data are provided as a Source data file.

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