Fig. 5: Immunogenicity of cocktail and mosaic E2E1-NP vaccines.

a Rabbits were immunized at weeks 0 and 4 with AMS3a E2E1-NP (same group as Fig. 3), a cocktail of six monovalent E2E1-NPs or mosaic E2E1-NP. Antibody responses were measured at week 6. b Binding titers against a hexavalent mix of E2E1-foldon trimers (left) or hexavalent mix of HVR1 peptides (middle). Both mixes are sequence-matched to the cocktail and mosaic vaccines. Right: ratio between the HVR1 titer and E2E1-foldon titers. c Spider plots of the individual rabbit ID₅₀ titers (thin lines) and their geometric mean titers (GMT) (bold lines) against vaccine-matched HCVpp. d Spider plots of the individual rabbit ID₅₀ titers (thin lines) and the GMT (bold lines) against mismatched HCVpp. e Comparison of the GMT values from (c) and (d). Groups were compared using Friedman test and Dunn’s post-test. f The global geometric mean titer (GGMT) against the six vaccine-matched (left) or ten mismatched HCVpp (middle). Ratio of the GGMT against the mismatched and vaccine-matched HCVpps (right). Each dot represents a single rabbit. g Comparison of the NAb responses induced by AMS3a E2, E2E1-I53-50A and E2E1-NP (from Fig. 3) with those induced by cocktail E2E1-NP and mosaic E2E1-NP (Fig. 5). Each dot represents a single rabbit serum. GGMT was calculated over sixteen HCVpp strains. Breadth was defined as the number of viruses neutralized with an ID₅₀ titer >40. Horizontal lines in (b), (e), (f) indicate the median values. Significant differences between groups in (b), (e), (f) were determined using a Kruskal–Wallis test followed by Dunn’s post-test, p-values are indicated; n = 6 rabbit sera per group (b and f) or n = 16 geomean ID₅₀ titers from 6 rabbit sera per group (e). P-values for significant differences are indicated on top of the graphs. Source data are provided as a Source data file.