Fig. 1: Single-cell transcriptome atlas of the human testis in health and T2DM. | Nature Communications

Fig. 1: Single-cell transcriptome atlas of the human testis in health and T2DM.

From: Targeting APLN/APJ restores blood-testis barrier and improves spermatogenesis in murine and human diabetic models

Fig. 1

a Schematic illustration of the experimental and analysis workflow. b UMAP and clustering analysis of single-cell transcriptome data from normal spermatogenesis human testis (n = 2810), integrated with single-cell transcriptome data of two Type 2 diabetes mellitus (T2DM)’ testes (DM patient 1 (DM1), n = 441; DM patient 2 (DM2), n = 459). Each dot represents a single cell and is colored according to its donor of origin. c UMAP plots showing the cell colors by the identified cell clusters between normal and diabetic patients. d Histogram showing the number of (left) upregulated differential expression genes and (right) downregulated differential genes from the normal and diabetic patients single-cell RNA sequencing data. The blue histogram represented total differential genes, and the red represented metabolic genes. e Enriched GO terms in the upregulated and downregulated metabolism-related genes of testicular cells from diabetic versus normal. The statistical test is a hypergeometric test. f Metabolic pathway activities in the testicular cell types including spermatogonia (SPG), spermatocyte (SPC), spermatid (SPD), and Sertoli (ST) cells from the normal and diabetic single-cell RNA sequencing data.

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