Fig. 7: Hepatic SENP1 deficiency aggravates RIPK1-driven liver damage in HFD-induced NAFLD.
From: SENP1 prevents steatohepatitis by suppressing RIPK1-driven apoptosis and inflammation
a Body and liver weight of 6 months old male mice with indicated genotypes after feeding with high-fat-diet (HFD) for 4 months (n = 7 for Senp1f/f, n = 6 for Senp1f/f;Alb-Cre and Senp1f/f;Alb-Cre;Ripk1D138N/D138N). b Serum levels of ALT and AST of mice from a (n = 6 for Senp1f/f and Senp1f/f;Alb-Cre, n = 5 for Senp1f/f;Alb-Cre;Ripk1D138N/D138N). c TUNEL assay was performed on liver sections of mice from a. Graph depicting numbers of TUNEL+ cells on sections of indicated genotypes. HE staining (d) and Oil Red O staining (e) of liver sections of mice from a. Graph depicting percentage of lipid content on liver sections of indicated genotypes (e). f Immunohistochemistry of CD45 of liver sections of mice from a. Arrows indicates infiltration of macrophages. Graph depicting numbers of CD45+ foci on liver sections of indicated genotypes. g Quantitative RT-PCR analysis of the mRNA expression of cytokines and chemokines in livers of mice from a. h Representative images of Masson’s trichrome stained (MTS) liver sections of mice from a. Graph depicting percentage of fibrosis area on liver sections of indicated genotypes. i Quantitative RT-PCR analysis of the mRNA expression of fibrogenic parameters in livers of mice from a. n = 7 mice for Senp1f/f, n = 6 mice for Senp1f/f;Alb-Cre, and n = 5 mice for Senp1f/f;Alb-Cre;Ripk1D138N/D138N (c-i). Data are represented as mean ± s.e.m. (a–c, e–i). One-way ANOVA, post hoc Dunnett’s test (a–c, e–i). Source data are provided as a Source Data file.
