Fig. 1: In situ ribosome structure of D. discoideum.
From: Structures of the eukaryotic ribosome and its translational states in situ
a Cryo-FIB milled lamella SEM view (top) and FIB view (bottom) of a D. discoideum cell. The lamella front in the FIB view is indicated by the white arrow. b Cryo-TEM overview of the same lamella, rotated by ~180° compared to (a top view), white box indicates the area shown in c. c Higher magnification cryo-TEM overview showing the subcellular context, white box indicates the area of tilt series acquisition. d Virtual slice of one out of 125 tomograms (dataset 1) and from the area shown in c, rotated clockwise by 90°. e Virtual slice of one out of 98 tomograms (dataset 2) at higher magnification. f Subtomogram average of the 80S D. discoideum ribosome obtained from dataset 2. g Corresponding local resolution map. Scale bars: 2 µm in a and b, 1 µm in c, and 100 nm in d and e.
