Fig. 3: Either IDR or CC4–6 can mediate PodJ phase separation in vitro.
From: Phase separation modulates the assembly and dynamics of a polarity-related scaffold-signaling hub
a Droplet formation for the truncated YFP-PodJ_N proteins. Images were taken within 15 min after loading 1, 2.5, 5, or 10 µM YFP-PodJ_N and its variants on the glass pad at 25 °C. b Summary of droplet formation by YFP-PodJ_N and the variants in panel a. c Comparative FRAP analysis reveals a faster fluorescence recovery for YFP-PodJ_N droplets than for YFP-PodJ_N(ΔCC1–3) droplets. The recovery curves for each sample were generated by averaging the signals of YFP-PodJ_N droplets (n = 6) from three independent experiments, and the representative droplets are shown. The fluorescence intensity of the pre-bleached droplets was normalized as 100%. Data are means ± SEM. All scale bars, 5 μm. Source data are provided in the Source Data file.
