Fig. 4: Creb5 is expressed in a reciprocal pattern to Wnt5a in the perichondrium.
From: Creb5 coordinates synovial joint formation with the genesis of articular cartilage
Gene expression assayed by RNAscope FISH in the forelimb of a WT E14.5 mouse embryo (a) or the knee of an E14.5 Creb5Δ9/Δ9 embryo or control littermate (b). In WT/control limbs, the articular perichondrium (yellow arrows) and metaphyseal/diaphyseal perichondrium (white arrows) are indicated. In Creb5Δ9/Δ9 mutant limb, the perichondrial cells expressing both Wnt5a and mutant Creb5Δ9/Δ9 transcripts (white arrowheads) are indicated. Similar results were obtained in at least three independent biological repeats. Scale bar equals 200 microns.
