Fig. 5: Misexpression of iCreb5-HA(Prx1-Cre) inhibits perichondrial expression of Wnt5a, Crabp1 and Runx2.
From: Creb5 coordinates synovial joint formation with the genesis of articular cartilage
Gene expression assayed by RNAscope FISH (a, b) or FISH (c) in serial sections of indicated limbs of either E14.5 Prx1-Cre-iCreb5 embryos or control littermates. In control limbs, the articular perichondrium (yellow arrows) and metaphyseal/diaphyseal perichondrium (white arrows) are indicated. In Prx1-Cre-iCreb5 limbs, the iCreb5-HA(Prx1-Cre)+, Wnt5a−/Crabp1− perichondrium (yellow arrowheads) is indicated. Femur (F) and Tibia (T) are indicated. Right-most panel of c displays immunofluorescent staining for Creb5 and DAPI in serial section of the same hindlimb analyzed by FISH. Red fluorescent signal in ectoderm is non-specific. Similar results were obtained in either 5 (a) or 2 (b, c) independent biological repeats. Scale bar equals 200 microns.
