Fig. 6: Misexpression of iCreb5-HA(Prx1-Cre) blocks growth plate formation and phenocopies aspects of Wnt5a loss of function.
From: Creb5 coordinates synovial joint formation with the genesis of articular cartilage
a Alcian Blue/Alizarin Red whole mount staining and b, c Von Kossa/Alcian Blue staining of indicated limbs from either P0 Prx1-Cre-iCreb5 mice or control littermates. Femur (F), Resting zone chondrocytes (R), Proliferating zone chondrocytes (P) and Hypertrophic zone chondrocytes (H) are indicated. Scale bar equals 100 microns in c. d, e Gene expression assayed by RNAscope FISH in E14.5 forelimbs of either control littermates, Wnt5aΔexon2/Δexon2 embryos or Prx1-Cre-iCreb5 embryos. Similar results were obtained in either 7 (a), 2 (b, c, e) or 3 (d) independent biological repeats. Scale bar equals 200 microns, unless otherwise indicated.
