Fig. 3: Immunoblot evaluation of ChR expression levels in Chlamydomonas reinhardtii. | Nature Communications

Fig. 3: Immunoblot evaluation of ChR expression levels in Chlamydomonas reinhardtii.

From: Gating and ion selectivity of Channelrhodopsins are critical for photo-activated orientation of Chlamydomonas as shown by in vivo point mutation

Fig. 3

a Protein immunoblotting of wild-type gamete cells, C1 and C2 strains, the double knock-out ΔC1ΔC2 and point mutants using anti-ChR1 antiserum and secondary HRP-conjugated antibody for chemiluminescence detection. An ATP synthase subunit B (AtpB) antibody (53 kDa) was used as a loading control. b ChR1 expression levels relative to the loading control AtpB. c Protein immunoblotting of the wild-type gamete cells, C1 and C2 strains, the double knock-out ΔC1ΔC2 and mutants using anti-ChR2 antiserum and secondary HRP-conjugated antibody for chemiluminescence detection. d ChR2 expression levels relative to AtpB. Lines represent the mean value. N = 2 independent experiments. Source data are available as a Source Data file.

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