Fig. 4: Phototactic response of Chlamydomonas reinhardtii. | Nature Communications

Fig. 4: Phototactic response of Chlamydomonas reinhardtii.

From: Gating and ion selectivity of Channelrhodopsins are critical for photo-activated orientation of Chlamydomonas as shown by in vivo point mutation

Fig. 4

a Change of orientation in a suspension of 1 × 106 cells/ml containing the wild-type, C1 and C2 strains, the double knock-out ΔC1ΔC2 and point mutants. The cells were illuminated with 470-nm light for 6 s (blue bar) perpendicular to the monitoring light. 100 % light intensity corresponds to 1.75 × 1019 photons·m−2s−1. The photophobic response is marked as STOP. b Rate of directional change is plotted versus light intensity. c Photosensitivity normalized to the mean irradiance value (3.05 × 1015 photons·m-2s-1) that wildtype cells needed to perform phototaxis. Lines and bars represent the mean value ± S.E. N = 3 independent experiments. Source data are available as a Source Data file.

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