Fig. 5: Electrophysiological characterization of the calcium permeation in C2, C1C2 and derived mutants. | Nature Communications

Fig. 5: Electrophysiological characterization of the calcium permeation in C2, C1C2 and derived mutants.

From: Gating and ion selectivity of Channelrhodopsins are critical for photo-activated orientation of Chlamydomonas as shown by in vivo point mutation

Fig. 5

a Representative photocurrent traces of the denoted variant, recorded from −80 to +40 mV in 20 mV steps under different extracellular buffer conditions in ND7/23 cells. Red traces depict the current at 0 mV holding voltage. b Zoom in on the currents in high [CaCl2]e at pHe 9. The green dotted line indicates net-zero photocurrent. Inward calcium-driven currents are highlighted in violet.

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