Fig. 4: NMPNs induce Pt-DNA adducts formation and oxidative cleavage of DNA strand. | Nature Communications

Fig. 4: NMPNs induce Pt-DNA adducts formation and oxidative cleavage of DNA strand.

From: A nuclease-mimetic platinum nanozyme induces concurrent DNA platination and oxidative cleavage to overcome cancer drug resistance

Fig. 4

a The release of Pt ions from NMPNs at different time points under neutral conditions after the discharge of mPEG5K-AC-CA. b The agarose gel electrophoresis of DNA treated with different concentrations of NMPNs. c Schematic illustration of Pt ion binding to DNA backbone. d Schematic illustration of ROS coordinating with Pt ion and causing oxidative DNA cleavage. e Comparison of hydrolysis and desorption process of complete DNA double-stranded fragments (upper) and DNA double-stranded fragments attacked by ROS (lower). The hydrolysis energy of DNA strand fragments attacked by ROS is −4.66 eV, lower than that of the complete double-stranded DNA (3.00 eV). Besides, the desorption energy of the unpaired base strand in ROS-attacked DNA is 3.05 eV, which is also lower than that of the bases with pairs in complete double-stranded DNA (9.22 eV). All data are presented as means ± SEM, n = 3 independent experiments. Source data are provided as a Source Data file.

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