Fig. 3: Crystal structures of nb.F3 and nb.E8 in complex with CaVβ1.
a Cartoon diagram of the nb.F3:CaVβ2a complex. CaVβ2a SH3 (helices (dark green) and β-strands (light green)) and NK (helices (dark blue) and β-strands (cyan)) domains are indicated. Nb.F3 (yellow) is shown with a semi-transparent surface. Nb.F3 Site 1 and Site 2 binding regions are indicated by the red and orange ovals, respectively. b Cartoon diagram of nb.F3:nb.E8:CaVβ1b complex. SH3 (helices (green) and β-strands (lime)) and NK (helices (purple) and β-strands (violet)) domains are indicated. Nb.F3 (yellow) and nb.E8 (yellow orange) are shown with a semi-transparent surface. Site 1 and Site 2 binding regions are indicated by the red and orange ovals, respectively. Light green oval denotes nb.E8 binding site. Lower panel shows view from the SH3 domain N-terminus as indicated by the arrows. ABP indicates AID binding pocket location in (a) and (b), (c) View of nb.F3:CaVβ2a Site 1 interactions. d View of nb.F3:CaVβ2a Site 2 interactions. e View of nb.E8:CaVβ1b interactions. Red ovals indicate CaVβ sites that differ among isoforms. Complementarity determining regions (CDRs) are colored similarly in all panels: CDR1 (purple), CDR2 (red), CDR3 (magenta). In (c–e) nanobody residues are in black, CaVβ residues are labeled in grey italics.
