Fig. 3: ocEAn, a tool to visualize metabolic changes in cancer cells.
a Representative scatter plot generated using ocEAn for BCAT1 in the indicated comparisons. Metabolites upstream and downstream of BCAT1 directly or indirectly linked to reaction are indicated in two separate plots, one (on top) for conversion of leucine in ketoisocaproic acid (KIC), the other (on the bottom) for the transamination of α-ketoglutarate (aKG) to glutamate. The dot size represents the multiplication of the t-value with the weighted distance index (distance index being the number of the x-axis). y-axis reports the t-value of the abundances for the metabolites indicated in BCAT1 footprint including if they are accumulated or depleted upstream or downstream. The most relevant metabolites are highlighted in green. For the scatter plot generation, the methymalonylcarnitine+succinylcarnitine metabolite was annotated as methylmalonylcarnitine only. b Proportion of total pool of the indicated labeled metabolites originating from 13C leucine + isoleucine in all renal cells at the indicated time points. Data represent the mean of 5 independent cultures ± SD. p-values were calculated using one-way ANOVA with multiple comparisons and indicated in the graph for the comparisons HK2 vs. other biological groups at the given time point.
