Fig. 2: Factors that contribute to Narta efficiency.
From: Gene activation guided by nascent RNA-bound transcription factors
a Schematic diagram of different knockin fragments and two different MS2 positions (in the intron or UTR region). b Representative images to show HSPB1 expression under various conditions, including different BFP tagging strategies illustrated in a. Scale bar, 10 μm. c Quantifications of protein expression based on fluorescent imaging under different conditions as shown in a. Each dot represents a single cell. n = 90 cells. d Schematic of the components to mediate Narta activation by PCP/PP7 or MCP/MS2. e Quantitative analysis of the protein expression level of endogenous reporters based on fluorescent imaging. Each dot represents a cell. n = 100 cells. f Schematic construction designs for testing dCas13-mediated Narta activation. g Performance of dCas13-mediated Narta activation as measured by quantitative imaging of endogenous reporters. Each dot represents a single cell. n = 100 cells. Data in c, e, g are shown as mean ± s.e.m. P values were calculated by two-tailed Student’s t-test. Source data are provided as a Source Data file.
