Fig. 2: MSEW alters activity levels and vHIP theta oscillations depending on the context. | Nature Communications

Fig. 2: MSEW alters activity levels and vHIP theta oscillations depending on the context.

From: The estrous cycle modulates early-life adversity effects on mouse avoidance behavior through progesterone signaling

Fig. 2

a Timeline of experiment. b Schematic of recording in home cage. c Locomotion in the home cage is increased in MSEW mice in diestrus (F1,8 = 7.132, p = 0.0283; Control-MSEW Diestrus p = 0.0226; Control n = 9, MSEW n = 10). d Climbing in the home cage is increased in MSEW mice in diestrus (F1,10 = 5.112, p = 0.0473; Control-MSEW Diestrus p = 0.0027; Control n = 8, MSEW n = 10). e vHIP theta power is increased in MSEW mice in diestrus (F1,10 = 7.238, p = 0.0238; Control-MSEW Diestrus p = 0.0090; Control n = 9, MSEW n = 10). f Power spectra from diestrus vHIP LFPs during home cage immobility in control and MSEW mice, inset graph shows vHIP theta power is higher in diestrus MSEW mice during immobility (t14 = 3.384, p = 0.0045; Control n = 7, MSEW n = 9). g vHIP gamma power is higher in control diestrus compared to MSEW diestrus (F1,11 = 11.36, p = 0.0063; Control-MSEW Diestrus p = 0.0012; Control n = 8, MSEW n = 10). h Schematic of recording in open field. i Locomotion in the open field center is reduced in MSEW mice during diestrus (F1,26 = 4.489, p = 0.0438; Control-MSEW Diestrus p = 0.0462; Control n = 7, MSEW n = 10). j Grooming in the open field is reduced in MSEW mice during diestrus (F1,11 = 11.25, p = 0.0064; Control-MSEW Diestrus p = 0.0450; Control n = 7, MSEW n = 10). k vHIP theta power is higher in the open field in MSEW mice (Estrous: F1,25 = 9.068, p = 0.0059; Control n = 7, MSEW n = 10). l Power spectra from diestrus vHIP LFPs during open field immobility, inset bar graph shows vHIP theta power is higher in diestrus MSEW mice during immobility (t11 = 2.344, p = 0.0389; Control n = 6, MSEW n = 7). m vHIP gamma power is reduced in MSEW mice (Estrous: F1,14 = 13.09, p = 0.0028; Control n = 7, MSEW n = 9). MSEW = maternal separation early weaning; arb. units = arbitrary units. *p < 0.05, mixed-effects model repeated measures (MSEW x Estrous) followed by Šidák post hoc tests (c, d, e, g, i, j); two-sided unpaired-tests (f, l). Data are presented as mean values + SEM for error bars (c-g; i-m) and ± SEM for bands (f, l). Images in b, h were created using BioRender.com. Source data are provided as a Source Data file.

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