Fig. 3: PEAC-seq identified DNA translocations relevant to CRISPR genome editing.

a Signal tracks of one PEAC-seq site with unexpected upstream signals from the F-primer amplicon. Dashed gray bar: cutting site; Earthy yellow peak: expected signals from the F-primer; Pink peak: unexpected signals from the F-primer. b Proposed models of the generation of unexpected upstream signals. Both the Receiver site and the Donor site could generate DSBs and proximal to each other within the nucleus. Models (i) and (ii) joined DSB ends from the same Receiver site. Models (iii), (iv), and (v) joined one donor DSB and one Receiver DSB. If the donor DSB carried the PEAC-seq insertion, the unexpected upstream signal would be observed at the Receiver Site. In the models, the gRNA location was set on the top strand. c The design of validation PCR to identify the genomic sequence of the Donor Sites. Two specific primers (Nest-F1 and Nest-F2) were designed upstream of the gRNA of the Receiver Site. The Nest-F1 and Nest-F2 were sequentially used with the downstream Tn5 primer, and two amplicons were generated. The second amplicons were sent for Amplicon-seq. d The translocation cases identified by PEAC-seq + Amplicon-seq. e Translocation scores of all sites were plotted. The red arrow indicated the Receiver Site in Fig. 3d. A DNA translocation score was calculated as “translocation reads number”/(“normal reads number” + “translocation reads number” + 10). Source data are provided as a Source data file.