Table 2 RT-PCR primers
Gene | Forward Sequence | Reverse Sequence |
|---|---|---|
Msi1 | 5′ - TGT CTG TGA ACA CCA CGG TG − 3′ | 5′ - CGT GAC AAA TCC AAA CCC TCT − 3′ |
MSI1 | 5′ - CAC CAA TGG GTA CCA CTG AA − 3′ | 5′ - ACT CGT GGT CCT CAG TCA GC − 3′ |
MYC | 5′ - AAT GAA AAG GCC CCC AAG GTA GTT ATC C − 3′ | 5′ - GTC GTT TCC GCA ACA AGT CCT CTT C − 3′ |
GAPDH | 5′ - TGA ACC ACC AAC TGC TTA GC − 3′ | 5′ - GGC ATG GAC TGT GGT CAT GAG − 3′ |
β-actin | 5′ - CCG AGC GTG GCT ACA GCT TC − 3′ | 5′ - ACC TGG CCG TCA GGC AGC TC − 3′ |
β-ACTIN | 5′ - TAT CCC TGT ACG CCT CT − 3′ | 5′ - AGG TCT TTG CGG ATG T − 3′ |
HIPK1 gRNA | 5′ - TCC CTT TTC ACT CCA AAT AGT TGT − 3′ | 5′ - ATG GTG AGC ACT ACC CTC CA − 3′ |
