Fig. 5: CA3-NR1 KO mice exhibited normal CA3 ensemble structure.
From: Hippocampus as a sorter and reverberatory integrator of sensory inputs
a Left, experimental design. Right, coronal section of the hippocampus with G-CaMP-expressed cells (green) in CA3 and immunostained with anti-RGS-14 (red). RGS-14 is a marker for CA2 and stacked dF/F images acquired through the microendoscope over entire recording sessions of imaging in the hippocampus. Similar expression pattern was confirmed at least 5 times independently. Scale bar, 500 μm. b Imaging data analysis scheme. In each cell, Ca2+ data is classified into nine sessions, and the calculated mean z-score is considered to represent responsiveness and sorted into CS-, US-, and Test-CS-responsive subpopulations. c Columns comparing the number of detected cells during CA3 imaging in control and CA3-NR1 KO mice. d Columns comparing percent of ensemble sizes in CS-, US-, and Test-CS-responsive subpopulations between control and KO mice. e Box plots comparing mean z-scores of long-term memory test sessions between genotypes in CS-, US-, and Test-CS-responsive subpopulations (two-tailed paired Wilcoxon signed-rank test: Ctrl CS cells Test-ACC to Test-CS, P = 0.012; KO US cells Test-ACC to Test-CS, P = 0.036; Ctrl Test-CS cells Test-ACC to Test-CS, P = 1.8E-31; KO Test-CS cells Test-ACC to Test-CS, P = 2.7E-28; Ctrl Test-CS cells Test-CS to Test-ITI, P = 1.9E-40; KO Test-CS cells Test-CS to Test-ITI, P = 2.5E-28). f Venn diagrams comparing and illustrating the overlapping and size of each ensemble in CA3. Columns comparing percent of overlapping ensemble sizes between CS-, US-, and Test-CS-responsive subpopulations between control and KO mice. Numbers in parentheses denote the number of (c, d, f) mice or (e) cells in each group used for the study. P values were determined using (c, d, f) an unpaired two-tailed t test or (e) a two-tailed Wilcoxon signed-rank test (*P < 0.05, ****P < 0.001). N.S., not significant (P > 0.05). Box plots represent median, first, and third quantiles, and minimum and maximum values. Graphs show means ± SEM. In graphs, circles represent individual animals. Detailed statistics are shown in Supplementary Data 1.
