Fig. 2: The N-terminus of AlgX is required for interaction with AlgK. | Nature Communications

Fig. 2: The N-terminus of AlgX is required for interaction with AlgK.

From: Structure of the AlgKX modification and secretion complex required for alginate production and biofilm attachment in Pseudomonas aeruginosa

Fig. 2

a Gel filtration chromatograms of AlgKPp (light green), AlgXPp (periwinkle), and the AlgKXPp complex (black) overlaid. This experiment was repeated independently one other time. b Coomassie-stained SDS-polyacrylamide gels corresponding to the indicated fractions (top) from the experiments in panel a. Gel images are outlined using the same color scheme as in panel a. This experiment was not repeated. c Gel filtration chromatograms of AlgKPp (light green), AlgXPp∆Nterm (periwinkle), and the two added together (black) overlaid. This experiment was repeated independently one other time. d Coomassie-stained SDS-polyacrylamide gel corresponding to the indicated fractions (top) from the experiments in panel c. Gel images are outlined using the same color scheme as in panel c. This experiment was not repeated. Molecular weight standards are indicated by the arrows: γ γ-globulin, O ovalbumin, M myoglobin, and V vitamin B12 with molecular weights 158, 44, 17, and 1.35 kDa, respectively. Source data are provided as a Source Data file.

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