Fig. 3: The AlgKX complex binds alginate. | Nature Communications

Fig. 3: The AlgKX complex binds alginate.

From: Structure of the AlgKX modification and secretion complex required for alginate production and biofilm attachment in Pseudomonas aeruginosa

Fig. 3

a Electrostatic surface representation of the AlgKXPp complex calculated by APBS Tools; contoured from +5 (blue) to −5 (red) kT/e. The box highlights the proposed pathway from the site of alginate acetylation by AlgXPp towards alginate export via AlgKPp. The dashed bracket represents the distance of the proposed pathway. Dotted black lines represent the boundary between AlgKPp and AlgXPp. Nickel (filled green circles) can be observed. b Representative ESI mass spectrum acquired for ammonium acetate (200 mM, pH 7.0) solutions of AlgKPp and AlgXPp (5 μM each protein) with GMGMGMG ligand (100 μM). AlgKPp ≡ AlgK, AlgXPp ≡ AlgX, AlgKXPp ≡ AlgKX. Ion signals specific for AlgKXPp complex with ligand bound are indicated by +L.

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