Fig. 6: CX3CR1– pvM share phagocytic functioning with CX3CR1+ pvM.
From: Deciphering the heterogeneity of the Lyve1+ perivascular macrophages in the mouse brain
a–c Cx3cr1GFP mouse brains were injected intra-ventricular with Dextran-Alexa647 (37 µm maximum intensity projection) (n = 3) and d–f Acetylated-LDL-Alexa594 (42 µm maximum intensity projection) (n = 3). Ten minutes after injection, mice were euthanized and brains dissected. Confocal analysis on brain sections shows Lyve1 (white), Dyes (red), and CX3CR1 (green). The unconventional pvMs phagocytosed the dyes (red arrows), as well as did conventional pvMs (white arrows) (b, c, e, f). g Tiled confocal acquisition of a Prox1mOrange2 brain section. Lyve1+ cells in green lined blood vessels stained for CD31 in blue. Lyve1+ cells in green penetrated the brain to the hippocampal fissure (hif) and all the way towards the third ventricle (3 V). (n = 4).
