Fig. 5: Spatiotemporally resolved TGT rupture.
From: Single-molecule characterization of subtype-specific β1 integrin mechanics
Time-resolved maps of TGT rupture induced by cell spreading (BHQ2-Cy3). Signal color is keyed by time (see color key). Time-lapse images were taken at 1 min intervals and pixelwise signal change was analyzed. See also Movie 5. The rupture signal, cumulated for a given time period, reflects integrin force transmission events (>Ttol). Two-minute cumulation is shown for cells spreading on RGD-54pN (a) and LDVP-33pN (b). Boxed regions are magnified. Panel a is at higher contrast than b to better show the spatial distribution of signals. Panel i directly compare intensity. The rupture signals (colored) were overlaid on RICM images (gray) in the first columns. c–h Rupture signals superimposed for 1 h for the indicated TGT. Scale bars, 10 µm. Representative results from multiple experiments. i TGT rupture rate for multiple cells (mean ± SE). Cells that ruptured less than 200,000 qTGT molecules were analyzed (n = 15, 18 cells from 3 independent experiments). Source data are provided as a Source Data file.
