Table 1 Comparison of genetically encoded serotonin sensors
From: Next generation genetically encoded fluorescent sensors for serotonin
Sensor | (ΔF/F0) % change | Kd | τON (ms) | τOFF = (ms) | |||||
|---|---|---|---|---|---|---|---|---|---|
10 nM | 100 nM | 1.6 µM | 3.2 µM | 100 µM | 1 mM | ||||
L-sDarken | – | – | – | −20 | −48 | −73 | 45 µM | 95 | 156 |
sDarken | −14 | −33 | −58 | −60 | −40 | Sat. | 127 nM | 43 | 323 |
H-sDarken | −8 | −51 | −62 | −67 | Sat. | Sat. | 57 nM | 57 | 324 |
GRAB5-HT110 | 70 | 240 | 280 | Sat | Sat | Sat | 22 nM | 200 | 3100 |
iSero13 | 15 | 20 | 30 | 75 | 750 | Sat | 390 µM | 0.5–10 (fast) 5–18 (slow) | 4 (fast) 150 (slow) |
PsychLight226 | 25 | 55 | 80 | Sat | Sat | Sat | 26.3 nM | 997 (fast) 3998 (slow) | |
