Fig. 3: Tbx2 patterns prosensory progenitors in the developing organ of Corti.
From: TBX2 specifies and maintains inner hair and supporting cell fate in the Organ of Corti
a–e Expression analysis of genes with increased expression in microarrays of E14.5 cochleae in which Tbx2 was ablated in prosensory cells (Sox2-Tbx2LOF) by tamoxifen administration at E12.5. a List of genes with increased expression in E14.5 Sox2-Tbx2LOF cochleae as detected by microarray analysis (n = 4). FC, fold change b–e RNA in situ hybridization analysis of selected candidates (b, c) on E14.5 (b) and E18.5 (c) cochlear sections of Sox2-Tbx2LOF embryos, and of Fgfr3 and its target genes Etv4 and Etv5 (d, e) on cochlear sections of E14.5 Sox2-Tbx2LOF (d) and E16.5 Sox2-TBX2ME (e) embryos. n = 3 for each genotype at each stage. Arrows in c point to Rnf182-expressing hair cells, arrowheads to pillar cells (PCs). Dashed lines in b, d show the position of the developing organ of Corti and the subdivision into the inner compartment (IC) and outer compartment (OC). Dotted circles in c, e mark the nuclei of hair cells. Scale bars: 30 µm. LER lesser epithelial ridge, GER greater epithelial ridge, OoC organ of Corti.
