Fig. 5: Brown adipocytic SIRT7 suppresses energy expenditure and thermogenesis in vivo.
a, b Data of indirect calorimetry experiments and body temperature from 12-week-old male Ucp1-Cre control and Sirt7 BAdKO mice. VO2 rates (a) and energy expenditure (b). p = 9.1E–36 in (a); p = 1.8E–04 (Dark), p = 3.8E–05 (Light), p = 3.6E–05 (Total) in (b). c Oscillation of body temperature in 20-week-old Ucp1-Cre control and Sirt7 BAdKO mice. p = 0.0399. d Real-time qPCR analysis of BAT-related genes in iBAT of 12-week-old male Ucp1-Cre control and Sirt7 BAdKO mice. p = 0.0245 (Prdm16), p = 0.0046 (Cebpb), p = 0.0009 (Fgf21), p = 0.0056 (Nrg4). e Western blot analysis of UCP1 in iBAT of 12-week-old male Ucp1-Cre control and Sirt7 BAdKO mice (left panel). Quantification of the UCP1 protein bands relative to GAPDH control is shown on the right side. p = 0.0192. f, g Data of indirect calorimetry experiments and body temperature from 20-week-old male Ucp1-Cre control and Sirt7 BAdKO mice at thermoneutrality. Energy expenditure (f) and body temperature at ZT10 (g). Data are presented as means ± SEM. All numbers (n) are biologically independent samples. Two-way ANOVA with Bonferroni’s multiple comparisons test (a, c); two-tailed Student’s t-test (b, d–g). *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.
