Fig. 6: SIRT7 cell-autonomously suppresses mitochondrial respiration via a reduction in the UCP1 protein level in brown adipocytes. | Nature Communications

Fig. 6: SIRT7 cell-autonomously suppresses mitochondrial respiration via a reduction in the UCP1 protein level in brown adipocytes.

From: SIRT7 suppresses energy expenditure and thermogenesis by regulating brown adipose tissue functions in mice

Fig. 6

a Oil red O staining of differentiated primary brown adipocytes from WT and Sirt7 KO mice (left panel, scale bar = 50 μm), and quantification of the stained oil red O (right panel). b Real-time qPCR analysis of BAT-related genes in the cells described in (a). c Western blot analysis of UCP1 in the cells described in (a) (left panel), and quantification of the UCP1 protein bands relative to GAPDH control (right panel). p = 0.0307. d Evaluation of the mtDNA copy number in differentiated primary brown adipocytes from WT and Sirt7 KO mice by determining the ratio of mtDNA to nDNA. e, f Mitochondrial stress test in the cells described in (a). Time course OCR (e) and quantification of mitochondrial respiration (f). p = 0.0247 (basal), p = 0.0239 (maximal). Data are presented as means ± SEM. All numbers (n) are biologically independent samples. *p < 0.05 by two-tailed Student’s t-test. Source data are provided as a Source Data file.

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