Fig. 2: Enzyme activities.

Heat map profiles of pH and temperature screening for hydrolytic activity on amorphous PET film by a diverse selection of 19 candidate enzymes and a positive control enzyme, LCC ICCG. The heat map gradient indicates the extent of measured product release up to 500 mg/L of total aromatic products after 96 h reaction time, and is reported as the average of reactions performed in triplicate (n = 3). Each heat map displays the reaction conditions utilized (citrate at pH 6.0, NaH₂PO₄ at pH 7.0, NaH₂PO₄ at pH 7.5, HEPES (H) at pH 7.5, bicine at pH 8.0, and glycine at pH 9.0), and reaction temperature (30, 40, 50, 60, or 70 °C). The heat maps for all other enzymes tested on amorphous PET film are shown in Supplementary Fig. 3. Source data are provided as a Source Data file.