Fig. 4: Activity of bacterial VTDs. | Nature Communications

Fig. 4: Activity of bacterial VTDs.

From: A widely distributed family of eukaryotic and bacterial deubiquitinases related to herpesviral large tegument proteins

Fig. 4

a, b Reaction of Wc-VTD1 (a) and Wc-VTD2 (b) with Ub and Ubl activity-based probes. Asterisks mark the shifted bands after reaction. c, d Activity of Wc-VTD1 and Wc-VTD2 against Ubiquitin-AMC (c) and NEDD8-AMC (d). The RFU values are the means of triplicates. e, f Linkage specificity analysis of Wc-VTDs. A panel of homotypic di-ubiquitin chains was incubated with Wc-VTD1 (e) or Wc-VTD2 (f) for the indicated time points. Source data are provided as a Source Data file.

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