Fig. 6: Activity of fungal and protozoan VTDs. | Nature Communications

Fig. 6: Activity of fungal and protozoan VTDs.

From: A widely distributed family of eukaryotic and bacterial deubiquitinases related to herpesviral large tegument proteins

Fig. 6

a–c Reaction of Nf-VTD (a), Si-VTD (b) or Sl-VTD (c) with Ub and Ubl activity-based probes. d, e Activity of Nf-VTD against Ubiquitin-AMC (d) and NEDD8-AMC (e). The RFU values are the means of triplicates. f, g Activity of Si-VTD against Ubiquitin-AMC (f) and NEDD8-AMC (g). The RFU values are the means of triplicates. h, i Activity of Sl-VTD against Ubiquitin-AMC (d) and NEDD8-AMC (e). The RFU values are the means of triplicates. j–l Linkage specificity analysis of eukaryotic VTDs. A panel of homotypic di-ubiquitin chains was incubated with Nf-VTD (j), Si-VTD (k) or Sl-VTD (l) for the indicated time points. Source data are provided as a Source Data file.

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