Fig. 2: Application of PhaseScan to a variety of condensate systems.
From: Biomolecular condensate phase diagrams with a combinatorial microdroplet platform
a Phase diagram of EGFP-tagged FUSG156E vs. polyU RNA concentration. N = 2096 droplets. b Phase diagram of FUSG156E condensation as a function of protein and salt concentration. N = 2625 droplets. c Phase diagram of EGFP-tagged G3BP1 vs. PEG 6000 concentration. N = 2549 droplets. d Phase diagram for polyA RNA-mediated phase separation G3BP1. N = 3077 droplets. e Phase diagram for coacervation-condensation of rotavirus proteins NSP2 and NSP5. N = 1672 droplets. f Phase diagram for polyA RNA-mediated phase separation of SARS-CoV-2 N protein. N = 1599 droplets. Red and blue data points in scatter plots correspond to individual microdroplets classified as phase separated or homogeneous, respectively. The heat map corresponds to the probability of phase separation as determined by an SVM classifier trained on the droplet scatter plot. Source data are provided as a Source Data file.
