Fig. 2: Properties of purified nanobodies exhibiting varying degrees of polyreactivity.
From: An in silico method to assess antibody fragment polyreactivity
a Spodoptera frugiperda (Sf9) insect cell PSR staining of single nanobodies isolated from FACS sorts. Data are mean +/− SEM of three independent biological experiments performed in technical triplicate. Polyreactivity levels are normalized with respect to the highest clone (Nb F02’). b CDR sequences of isolated nanobodies. c Direct ELISA assays measured the apparent EC50 (EC50APP) of five index set members and nanobody AT118i4h32 to the specified reagents. Non-specific binding, indicated by low EC50APP values, correlates with strong binding to PSR. ELISA data are representative of two independent experiments, each performed in technical triplicates.
