Fig. 4: Nr6a1 suppresses posterior Hox gene expression.
From: Nr6a1 controls Hox expression dynamics and is a master regulator of vertebrate trunk development
A Quantitative PCR analysis of posterior Hox gene expression within tailbud tissue isolated from TCreERT2 controls, TCreERT2;Nr6a1+/flx and TCreERT2;Nr6a1flx/flx embryos at E9.5, n = 3 biologically independent samples per genotype. Statistical analysis of differences between genotypes was performed using an unpaired two-sample Welch’s t test, p values are provided in the figure. Source data are provided as a Source Data file. B Whole mount in situ hybridisation revealed a spatial expansion of Hoxb13 expression in TCreERT2;Nr6a1+/flxembryos compared to wildtype, at both E9.5 and E10.5. Red arrow at E9.5 indicates ectopic Hoxb13 expression. TBM = tail bud mesoderm; NT = neural tube.
