Fig. 6: Gdf11 signalling terminates Nr6a1-dependent trunk elongation.
From: Nr6a1 controls Hox expression dynamics and is a master regulator of vertebrate trunk development
A Skeletal preparation of E18.5 embryos, lateral view, revealed a dose-dependent rescue of Gdf11−/− thoraco-lumbar expansion following conditional deletion of Nr6a1 alleles. The number of biologically independent samples assessed: Nr6a1+/+;Gdf11−/− n = 9; Nr6a1+/−;Gdf11−/− n = 5; Nr6a1−/−;Gdf11−/− n = 7. T = thoracic, L = lumbar. B Quantification of thoracic, lumbar and sacro-caudal vertebral number across genotypes. Raw data is presented in the upper plots. Mean differences were calculated relative to Gdf11−/−;Nr6a1+/+ and presented in the lower plots as bootstrap sampling distributions. The mean difference for each genotype is depicted as a black dot and 95% confidence interval is indicated by the ends of the vertical error bar.
