Fig. 5: Effects of native HSP70/GRP78 inhibition with mAbs on NASH hallmarks. | Nature Communications

Fig. 5: Effects of native HSP70/GRP78 inhibition with mAbs on NASH hallmarks.

From: Upper gut heat shock proteins HSP70 and GRP78 promote insulin resistance, hyperglycemia, and non-alcoholic steatohepatitis

Fig. 5

a–c Representative Hematoxylin and Eosin (H&E) (a), Oil Red O (ORO) (b), and Picro Sirius Red (c) staining of liver sections from rats fed HFD and infused with monoclonal antibodies against HSP70 or GRP78 or antibody isotype. d Gene expression of liver inflammation and fibrosis markers as well as key enzymes involved in hepatic de novo lipogenesis. e Plasma low-grade inflammation markers assessed by Luminex. f Gene expression of key genes involved in intestinal permeability. g Time course and AUC of in vivo intestinal permeability assessed by measuring Fluorescein IsoThioCyanate Dextran 4000 Da (FITC-DXT) in the systemic circulation after an oral gavage of FITC-DXT (500 mg/kg body weight). h–j, Hepatic (h), skeletal muscle (i), and jejunal (j) expression of key proteins involved in Unfolded Protein Response (UPR). Magnification ×20. Scale bar: 0.10 mm. Data are presented as mean value ± SEM of n = 10 biologically independent animals. Statistical significances were calculated by one-way Anova with Bonferroni’s correction for multiple comparisons. Source data are provided as a Source Data file.

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