Fig. 4: Comparison of BS-IDT and BS-DPC microscopy.
a–c BS-IDT cold and chemical 3D RI reconstruction results sectioned at 0 µm depth. Sample: fixed mitotic bladder cancer cells in D2O PBS. d–f BS-DPC cold and chemical 2D phase reconstruction results of the same sample. For images in “a–c” and “d–f”, each row corresponds to the same mid-IR radiation. g, i are line profiles extracted from the chemical imaging results of BS-IDT and BS-DPC. g Yellow-color profile line is extracted from the yellow dash-line area of “b”. Blue-color profile line is extracted from the blue-dash-line area of “e”. i Red-color profile line is extracted from the red dash-line area of “c”. Green-color profile line is extracted from the green dash-line area of “f”. h, j are line profiles extracted from the “cold” imaging results of BS-IDT and BS-DPC. h Yellow-color profile line is extracted from the yellow dash-line area of “a”. Blue-color profile line is extracted from the blue-dash-line area of “d”. j Red-color profile line is extracted from the red dash-line area of “a”. Green-color profile line is extracted from the green dash-line area of “d”.
