Fig. 5: BS-IDT imaging and fingerprint spectroscopy on C. elegans.

a–j Sample: fixed C. elegans (daf-2 (e1370) mutant strain) immersed in D₂O PBS. From “a” to “f”, images in each column are from the same depth. Images in each row are under the same mid-IR laser illumination conditions. The scale bar in “a” is applicable to “a–c”. The scale bar in “d” is applicable to “d–f”. a, d Depth-resolved cold imaging results. b, e Depth-resolved protein imaging results at 1657 cm⁻¹ wavenumber. c, f Depth-resolved lipid imaging results at 1745 cm⁻¹ wavenumber. d–f Cold, protein, and lipid imaging results of dash-line square area in “a”. g–i 3D rendering of the C. elegans shown in “a”, “b”, and “c”. j is the 3D overlay of “h” and “i”. k Mid-IR Fingerprint spectra extracted from protein and lipid-rich areas as well as areas without worms. The chemical images on the right side of the plot show the areas of interest that are used to extract the spectra.